What is the wavelength for FITC?
What is the wavelength for FITC? 475 to 650 nm FITC emits fluorescence from 475 to 650 nm, peaking at 525 nm, which falls in the green spectrum. What does the fluorescence spectrum represent? The fluorescence excitation spectrum characterizes the electron distribution of the molecule in the ground state. Excitation is equivalent to absorption since upon absorption, the molecule reaches the excited state Sn. What do fluorescent dyes stain in fluorescent microscopy? Direct count using fluorescent dyes The most widely used fluorescent dye for counting the number of bacterial cells is acridine orange which stains both living and dead cells...