How do you use polylysine?
How do you use polylysine? Dissolve 5mg poly-d-lysine, (70-150kd molecular weight), in 100ml sterile water to 50ug/ml.Add enough poly-d-lysine solution to cover culture surface.Incubate 1-24 hours.Remove polylysine solution and rinse the plate thoroughly.Let the plate dry.Store coated plates room temperature or 4-8°C. What is the role of poly D Lysine in cell culture? Poly-Lysine enhances electrostatic interaction between negatively-charged ions of the cell membrane and positively-charged surface ions of attachment factors on the culture surface. When adsorbed to the culture surface, it increases the number of positively-charged sites available for cell binding. What is PDL coating? Poly-D (or L)-Lysine coated...