What is growth factor reduced Matrigel?
What is growth factor reduced Matrigel?
Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, 10 mL, is suited for applications requiring a highly defined basement membrane preparation. Available in standard GFR, High Concentration (HC), and Phenol Red-free formats.
How long does Matrigel last in vivo?
one week
How long will a BD Matrigel matrix plug last in vivo? A BD Matrigel matrix plug will last for at least one week in vivo. Standard BD Matrigel matrix Standard 356234 354234 356235 General cell culture*. Phenol red-free 356237 General cell culture; assays that require color detection (e.g., fluorescence).
How much does Matrigel cost?
Products
| Product Number | Product Name | Price |
|---|---|---|
| Product Number354263 | Product NameCorning® Matrigel® Matrix High Concentration (HC), Growth Factor Reduced (GFR) *LDEV-free, 10 mL | Price $467.98 |
| Product Number354277 | Product NameCorning® Matrigel® hESC-Qualified Matrix, LDEV-free, 5 mL | Price $234.08 |
How long does Matrigel take to polymerize?
15 to 30 minutes
On ice, add 1.2 ml matrigel to tube. Carefully pipette up and down to distribute cells but not introduce bubbles into the matrigel. Transfer matrigel and cell mixture to pre-coated 35 mm dish. Place in 37oC incubator for 15 to 30 minutes and allow matrigel to polymerize.
What is the stiffness of Matrigel?
A stiffness value of 44 Pa has been reported for Matrigel matrix (high concentration product)22. Similarly, several publications have reported the stiffness values for Collagen I gels. The constructs became increasingly stiffer with the addition of Matrigel matrix (10% to 50% addition) with moduli ranging up to 10 kPa.
What is the purpose of Matrigel?
Matrigel has been used in various in vitro assays for angiogenesis, cell invasion, spheroid formation, organoid formation from a single cell, etc. In vivo Matrigel improves/promotes tumor xenograft growth and is used to measure angiogenesis, improve heart and spinal cord repair, increase tissue transplant take, etc.
Is Matrigel a hydrogel?
Other collagen-containing hydrogels have been used in cell studies, most notably Matrigel.
Do you need to dilute Matrigel?
To prevent incomplete gel formation for in vivo applications, do not dilute Corning Matrigel matrix to a final concentration below 4 mg/mL.
What does Matrigel consist of?
Matrigel™ is a reconstituted basement membrane derived from extracts of Engelbreth-Holm-Swarm mouse tumors. The basement membrane is structurally a thin layer of ECM sheets that consist primarily of collagen type IV, entactin, perlecan (heparan sulfate proteoglycan), and laminin.
What is BDBD Matrigel™ matrix growth factor reduced?
BD Matrigel™ Matrix Growth Factor Reduced (GFR) from BD Biosciences. The extracellular basement membrane plays a crucial role in angiogenesis, invasion and metastasis. The “Matrigel System” from BD Biosciences is one of the most widely used in vitro assays for such studies.
Why choose BDBD Matrigel hESC-qualified matrix?
BD Matrigel hESC-qualified Matrix has been qualified as mTeSR™1-compatible by StemCell Technologies, thus eliminating the need for time-consuming screening,
What are the typical endotoxin and protein concentrations for BD Matrigel matrix?
Typical protein concentrations for BD Matrigel Matrix are between 9-12 mg/ml. BD Matrigel Matrix High Concentration is 18-22 mg/ml. A lot-specific Certificate of Analysis is included in each BD Matrigel Matrix shipment noting exact endotoxin and protein concentrations.
Why use the growth factor-reduced (GFR) Matrigel?
The heterogeneity of Matrigel and its uncharacterized components can sometimes produce variability and background in experiments making critical interpretation of data difficult. The Growth Factor-Reduced (GFR) Matrigel is well-suited for studies which require a more defined and characterized reconstituted basement membrane than Matrigel.