What enzymes does Bacillus subtilis produce?

B. subtilis produces enzymes such as proteases and amylase.

Which bacteria produce xylanase?

2005) Bacterial genera, such as Bacillus, Cellulomonas, Micrococcus, Staphylococcus, Paenibacillus, Arthrobacter, Microbacterium, Pseudoxanthomonas, and Rhodothermus have been reported to produce xylanases (Subramanian and Prema 2000; Beg et al. 2001; Gupta et al. 2001; Chapla et al. 2012).

How do you make xylanase?

Industrial-scale xylanase production can be accomplished using SSF by fungi, mainly Aspergillus and Trichoderma. Fungi are potentially useful for xylanase production because they secrete enzymes into the medium, and their enzyme levels are, generally, much higher than those of yeasts and bacteria [59,60].

What is the substrate for xylanase?

Substrate specificity of the xylanase was found by using 1% xylan, cellobiose, starch, carboxy methyl cellulose (CMC), and p-nitrophenyl xylopyranoside and Avicel as substrates.

What is xylanase made of?

Xylanase is a group of enzymes consisting of endo-1,4-β-d-xylanases (EC 3.2. 1.8), β-d-xylosidases (E.C. 3.2. 1.37), α-glucuronidase (EC 3.2.

What is the substrate of xylanase?

How many types of shape do Bacillus subtilis have?

rod-shaped
subtilis cells are typically rod-shaped, and are about 4–10 micrometers (μm) long and 0.25–1.0 μm in diameter, with a cell volume of about 4.6 fL at stationary phase.

What is a xylanolytic bacteria?

A xylanolytic bacterium was isolated from the sediment of an aquarium. Based on the 16S rDNA sequence as well as morphological and biochemical properties the isolate was identified and denoted as Bacillus subtilis(B. subtilis) AQ1 strain.

Is it possible to clone the xylanase gene in E coli?

Cloning and expression of an endoxylanase gene from a localBacillus licheniformisinto E. coliusing an E. coliplasmid promoter have previously been conducted [8]. In this paper, we describe the cloning and efficient expression of a family 11 xylanase gene (xyn11) governed by its own promoter.

What is Bacillus subtilis aq1 strain?

Based on the 16S rDNA sequence as well as morphological and biochemical properties the isolate was identified and denoted as Bacillus subtilis(B. subtilis) AQ1 strain. An endoxylanase-encoding gene along with its indigenous promoter was PCR amplified and after cloning expressed in E. coli.

Where is endoxylanase found in E coli?

An endoxylanase-encoding gene along with its indigenous promoter was PCR amplified and after cloning expressed in E. coli. In E. colithe recombinant enzyme was found in the extracellular, in the cytoplasmic, and in the periplasmic fraction.