What is a bacterial pan-genome?

The pan-genome is a kind of reference genome that represents all genes in a collection of bacterial isolates. The pan-genome is usually defined for bacteria and viruses because they are highly recombinogenic and have a small genome and many isolates can be cloned and sequenced quickly.

How does reverse vaccinology work?

Reverse vaccinology uses the expressed genomic sequences to find new potential vaccines. Normal vaccines are created using the pathogenic organism. The term reverse refers to the use of expressed DNA over the purified proteins from the organism itself.

What is pan-genome approach?

In this context, a powerful approach called Pan-genome has been initiated and developed. This approach involves the genomic comparison of different strains of the same species, or even genus. Pan-genome consists of three parts: core genome; accessory or dispensable genome; and species-specific or strain-specific genes.

What is the difference between core genome and pan-genome?

The core-genome is the set of homologous genes that are present in all genomes of an analyzed dataset and the pan-genome is the set of all genes that are present in the analyzed dataset [16].

Is the pan-genome bigger than the core genome?

Another example of prokaryote pan-genome is Prochlorococcus, the core genome set is much smaller than the pangenome, which is used by different ecotypes of Prochlorococcus.

What is the pan-genome quizlet?

The core genome is a set of genes shared by all members of a species, while the pan genome includes the core genes as well as genes that are not shared by all members.

What is meant by vaccinology?

Vaccinology is defined as the science of vaccines, and historically includes basic science, immunogens, the host immune response, delivery strategies and technologies, manufacturing, and clinical evaluation.

What is the key to reverse vaccinology?

The basic idea behind reverse vaccinology is that an entire pathogenic genome can be screened using bioinformatics approaches to find genes.

Why we do pan-genome analysis?

Therefore, a pan-genome analysis helps to define which virulence genes are shared among all pathogenic species, as well as which genes are specific to one isolate. This has direct implications for understanding the evolution of pathogenic species.

Who proposed the term pan-genome?

Tettelin
1 Introduction Pan-genome is an approach that contributes to the research of bacterial pathogenesis. This terminology was proposed in 2005 in research with the bacterium Streptococcus agalactiae, by the researcher Tettelin and collaborators [1].