What color is Alexa Fluor 594?

What color is Alexa Fluor 594?

red
Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines.

What is phalloidin staining used for?

Introduction. Phalloidin is a highly selective bicyclic peptide used for staining actin filaments (also known as F-actin). It binds to all variants of actin filaments in many different species of animals and plants.

What does rhodamine phalloidin stain?

Phalloidins are used to stain F-actin in fixed cells. Fluorescent phalloidins only bind to the native quaternary structure of F-actin which provides a low background.

What is a phalloidin conjugate?

Phalloidin is a bicyclic heptapeptide isolated from the poisonous death cap mushroom, Amanita phalloides. Phalloidin conjugates exhibit similar affinity for all types and sizes of actin filaments, binding in a stoichiometric ratio of 1:1 (phallotoxin : actin) in both muscle and nonmuscle cells.

What Alexa 532?

Alexa Fluor 532 is a yellow-fluorescent dye with excitation ideally suited for the frequency-doubled Nd:YAG laser line. Alexa Fluor 532 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications.

What does phalloidin do to hepatocytes?

9.08. 3.5. 6 Phalloidin This poison causes cholestatic hepatotoxicity, characterized by cholestasis, because it is selectively taken up by hepatocytes where it is then able to bind to F-actin (Loranger et al.

How does phalloidin affect hepatocytes?

Phalloidin increases F-actin microfilament content and actin-directed immunofluorescence in hepatocytes in vivo and also increases actin polymerization and the stability of F-actin in vitro.

How do you stain with phalloidin?

Phalloidin Staining Protocol

1. Reconstitute phalloidin according to manufacturer’s directions.
2. Fix cells in the collagen gels with 3.7% (v/v) paraformaldehyde 10 min at room temperature.
3. Rinse 3 times in PBS.
4. Permeabilize cells with 0.1 -0.5% (v/v) Triton X-100 for 10 min at room temperature.
5. Rinse 3 times in PBS.

Can phalloidin stain live cells?

Phalloidin is toxic to living cells.

Is Alexa 555 red?

Alexa Fluor® 555 (AF555, Alexa 555) is a yellow-emitting synthetic fluorophore that can be excited by the 532 nm yellow laser and captured with a 575/36 nm bandpass filter.