What is solid phase microextraction method?

Solid phase microextraction, or SPME, is a solid phase extraction sampling technique that involves the use of a fiber coated with an extracting phase, that can be a liquid (polymer) or a solid (sorbent), which extracts different kinds of analytes (including both volatile and non-volatile) from different kinds of media.

What is microextraction method?

Microextraction is defined as an extraction technique where the volume of the extracting phase is very small in relation to the volume of the sample, and extraction of analytes is not exhaustive. From: Journal of Chromatography A, 2000.

Who invented solid phase microextraction?

Prof. Janusz Pawliszyn
Solid phase microextraction (SPME) is one of several important sample preparation techniques. SPME, invented in early nineties by Prof. Janusz Pawliszyn from the University of Waterloo in Ontario, Canada, integrates sampling, extraction, concentration and sample introduction into a single step.

What is Matrix solid phase dispersion?

Matrix solid phase dispersion (MSPD) is a technique widely used in sample preparation for the extraction of chemical constituents from fruits and plants, as well as for the extraction of organic contaminants from food and environmental samples.

What is liquid phase microextraction?

Liquid-phase microextraction is a miniaturized form of traditional liquid–liquid extraction in which the extracting organic phase is limited to a few microliters for extraction of target analytes.

What is solid phase extraction used for?

Solid phase extractions (SPEs) are routinely used in the sample preparation for quantifying analytes in biological fluids such as plasma and urine. This technique allows both removal of interfering biological matrix components and enhancing the concentrations of analytes in LC/MS samples.

What is single drop microextraction?

Single-drop microextraction (SDME) is often used to represent the acceptor phase where only a small microdroplet (0.5–2.0 µl) is suspended on the tip of a microsyringe; while liquid–liquid microextraction (LLME) is used when the acceptor phase is >10 µl [2].

Why solid phase microextraction SPME is a powerful sample preparation tool prior to mass spectrometric analysis?

SPME reduces the time necessary for sample preparation, decreases purchase and disposal costs of solvents and can improve detection limits. The SPME technique is ideally suited for MS applications, combining a simple and efficient sample preparation with versatile and sensitive detection.

What is a solid matrix?

solid matrix definition: An assembly of interconnected solid mineral grains by voids Definition of solid matrix: An assembly of interconnected solid mineral grains surrounded by voids [16]. ” />

What is the advantage of performing dynamic LPME?

Dynamic LPME This technique can be used for both SDME and HF-LPME. Dynamic LPME improves extraction rate compared with static solvent microextraction but the apparatus can be more complex.

What is solid phase extraction HPLC?

Solid phase extraction (SPE) is a technique designed for rapid, selective sample preparation and purification prior to the chromatographic analysis (e.g. HPLC, GC, TLC). In SPE, one or more analytes from a liquid sample are isolated by extracting, partitioning, and/or adsorbing onto a solid stationary phase.

Which is a main advantage of solid phase synthesis?

General advantages of solid phase synthesis are easy purification, rapid generation of linear peptide intermediates, and precedent in the synthesis of large peptides.