What is Effectene transfection reagent?

What is Effectene transfection reagent?

Effectene Transfection Reagent is a nonliposomal lipid reagent for DNA transfection into a broad range of cell types. Due to its low cytotoxicity, Effectene Transfection Reagent is highly suitable for transfection of primary cells and many sensitive cell lines.

What is FuGENE 6 transfection reagent?

FuGENE® 6 Transfection Reagent(a) is a nonliposomal reagent that transfects DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex.

What is a good transfection efficiency?

With cationic lipid-mediated transfection, generally 70–90% confluency for adherent cells or 5 × 105 to 2 × 106 cells/mL for suspension cells at the time of transfection provides good results.

What is TurboFect?

TurboFect Transfection Reagent is a sterile solution of a proprietary cationic polymer. TurboFect Reagent is ideal for transfection of a variety of cells, including primary and difficult-to-transfect cells.

How can I increase my transfection efficiency?

Improving the Success of Your Transfection

1. Transfect healthy, actively dividing cells at a consistent cell density.
2. Transfect using high-quality DNA.
3. Optimize the amount of DNA used to transfect cells.
4. Optimize the transfection reagent:DNA ratio.
5. Optimize cell number per well when transfected.

How do you measure transfection efficiency?

One general method for measuring transfection efficiency is to use a fluorescence microscope. The transfection efficiency is measured by counting the total number of observed cells and the number of cells that express fluorescence, and scoring these values.

How do you analyze a transfected cell?

Most methods for measuring protein expression level of your transfected cell will determine the total expression from a population of transfected cells. Measurement of total gene expression can be done through real-time quantitative PCR (real-time qPCR), western blot analysis, molecular imaging, and fluorometry.