Is IGEPAL the same as NP-40?

Nonidet P-40 and IGEPAL CA-630 are the same, those are merely different brand names. According to the manufacturer, Sigma-Aldrich, the two compounds are “chemically indistinguishable”. Both are octylphenoxy poly(ethyleneoxy)ethanol.

What is IGEPAL co 630?

IGEPAL® CO-630 is an alkylphenol ethoxylate that is majorly used as an anionic surfactant. It belongs to the IGEPAL series of surfactants, which have hydrophobic chains. Its properties include low surface tension, low sensitivity, and biocompatibility.

What is NP-40 substitute?

Applications Nonidet-P40 Br Substitute (NP-40 alternative) is used as nonionic surface-active agent. It is an effective emulsifier for solvents such as xylene. Furthermore in Biochemistry, Nonidet-P40 substitute (br) is a nonionic surfactant used in the isolation of membrane complexes.

What does NP-40 stand for?

Nonidet P-40
Nonidet P-40 (“Non-Ionic Detergent”) was originally manufactured and trademarked by the Shell Chemical Company, but was phased out of production in the early 2000s. Confusingly, biochemical protocols published between the 1960s and 2000s refer to Shell’s Nonidet P-40 as NP-40.

What is NP-40 used for?

NP-40 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or other solutions intended to extract and solubilize proteins. Surfact-Amps Detergent Solutions (10% w/v) provide unsurpassed purity, quality and stability.

What are nonylphenol ethoxylates?

NP/NPEs, also known as nonylphenols and nonylphenol ethoxylates, are nonionic surfactants, or detergent-like substances, with uses that lead to widespread release into aquatic environments. NP/NPEs are highly toxic to aquatic life.

How do you dilute NP-40?

Directions: 1) Mix 20 ml of NP-40 with 80 ml of ddH2O by stirring. 2) Store at 4°C. Note: Nonidet P-40 is chemically identical to Igepal-CA-630.

Does NP-40 denature proteins?

NP-40 (Nonidet P-40) and Triton X-100 are milder, nonionic detergents. They are good at solubilizing membrane proteins and for isolating cytoplasmic proteins. Proteins retain their native state in the presence of these detergents and protein-protein interactions can be preserved.

Is NP-40 a buffer?

NP40 Cell Lysis Buffer is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.