Can breast cancer be categorized with microarrays?

Microarray-based gene expression analysis can be used to analyze, display, and systemically classify the molecular heterogeneity observed among basal-like breast cancers.

What is the purpose of tissue microarray?

Tissue microarray is a practical and effective tool for high-throughput molecular analysis of tissues that is helping identify new diagnostic and prognostic markers and targets in human cancers.

What is tissue microarray construction?

Tissue microarrays (TMAs) are constructed by removing a core (tube shaped section) of tissue from a paraffin block (donor block) using a hollow needle and transferring this core to a predetermined position on a paraffin block (recipient block).

What is hybridization in a microarray analysis?

A microarray is a laboratory tool used to detect the expression of thousands of genes at the same time. The two samples are then mixed together and allowed to bind to the microarray slide. The process in which the cDNA molecules bind to the DNA probes on the slide is called hybridization.

What are FFPE samples?

FFPE is a form of preservation and preparation for biopsy specimens that aids in examination, experimental research, and diagnostic/drug development. A tissue sample is first preserved by fixing it in formaldehyde, also known as formalin, to preserve the proteins and vital structures within the tissue.

What is tissue core?

The Tissue Core (TC) is the central biorepository for Moffitt Cancer Center. The overall goal of the TC is to collect, process, store, and release high-quality, well-annotated biospecimens in support of research.

How is microarray performed?

To perform a microarray analysis, mRNA molecules are typically collected from both an experimental sample and a reference sample. The two mRNA samples are then converted into complementary DNA (cDNA), and each sample is labeled with a fluorescent probe of a different color.

How is microarray testing done?

How the Test is Done. A blood sample is preferred for microarray analysis. Sometimes spit (saliva) or a tissue sample (like skin) may be used. This test compares the patient’s sample to a normal control sample to find very small missing or extra chromosome pieces that cannot be seen under a microscope.

How do you get FFPE?

The FFPE Process After excision, the tissue is immersed for approximately eighteen to twenty-four hours in a 10% neutral buffered formalin. The tissue is then dehydrated using increasing concentrates of ethanol. Next, the tissue is embedded into paraffin to become FFPE blocks.

Is FFPE tissue infectious?

FSAP has determined that a select agent or regulated nucleic acids that can produce infectious forms of any select agent virus contained in a formalin-fixed paraffin-embedded (FFPE) tissue has been effectively inactivated if the FFPE process used is a recognized method (e.g. a previously published method shown to be …

Are primary breast carcinoma tumor tissues immunostained for Malay?

Two hundred and seventeen primary breast carcinomas tumor tissues were immunostained for ER, PR, HER2, CK5/6, EGFR, CK8/18, p53 and Ki67 using tissue microarray technique. All subtypes were significantly associated with Malay ethnic background (p=0.035) compared to other racial origins.

Can immunohistochemistry be used to identify the different subtypes of breast cancer?

Our studies suggest that IHC can be used to identify the different subtypes of breast cancer and all subtypes were significantly associated with race, mean tumor size, mean number of lymph node positive, histological grade and all immunohistochemical markers except EGFR and Ki67. Publication types

What are the most common subtypes of breast cancer?

The most common subtypes of breast cancers were luminal A and was significantly associated with low histological grade (p<0.000) and p53 negativity (p=0.003) compared to HER2+/ER-, basal-like and normal-like subtypes with high histological grade (p<0.000) and p53 positivity (p=0.003).