Does homologous recombination require Reca?
Does homologous recombination require Reca?
Main Text. Homologous recombination is an essential cellular process required, amongst other things, for the completion of DNA replication, the faithful repair of DNA damage and, in meiosis, for the genetic re-assortment that occurs during the production of gametes.
What is a function of RecA during homologous recombination?
During homologous recombination, the RecA nucleoprotein filament catalyzes the pairing and exchange of complementary DNA strands between homologous regions of DNA.
What does recombinase RecA do?
Recombinases are responsible for homologous recombination and maintenance of genome integrity. In Escherichia coli, the recombinase RecA forms a nucleoprotein filament with the ssDNA present at a DNA break and searches for a homologous dsDNA to use as a template for break repair.
What is heteroduplex in Holliday model?
In 1964, Robin Holliday proposed a model that accounted for heteroduplex formation and gene conversion during recombination. This occurs in a reciprocal manner to produce a heteroduplex regionderived from one strand from each parental duplex.
What is homologous recombination?
Homologous recombination is a DNA repair pathway of clinical interest due to the sensitivity of homologous recombination-deficient cells to poly-ADP ribose polymerase (PARP) inhibitors.
What is homologous recombination repair deficiency (HRD)?
The impairment of HRR activity is called homologous recombination repair deficiency (HRD) and is caused by different factors. Genes involved in the HRR pathway, such as BRCA1/2or PALB2,to repair DSB can be either mutated within the tumor (somatically) or germline, thus causing HRR impairment.
What phase of the cell cycle does homologous recombination repair double-strand breaks?
Whether homologous recombination or NHEJ is used to repair double-strand breaks is largely determined by the phase of cell cycle. Homologous recombination repairs DNA before the cell enters mitosis (M phase). It occurs during and shortly after DNA replication, in the S and G2 phases of the cell cycle,…
Is homologous recombination deficiency a useful biomarker for PARP inhibitors?
Homologous Recombination Deficiency (HRD) is associated with sensitivity towards PARP inhibitors (PARPi) and its determination is used as a biomarker for therapy decision making. Nevertheless, the biology of HRD is rather complex and the application, as well as the benefit of the different HRD biomarker assays, is controversial.