How do you make basic Fuchsin?

Dissolve 0.5 g basic fuchsin dye in 20 ml 95% ethanol. Dilute to 100 ml with distilled water. Filter if necessary with Whatman No. 31 filter paper to remove any undissolved dye.

Is Basic Fuchsin carcinogenic?

This substance is a potential carcinogen and should therefore be handled with extreme care. It is toxic and an irritant. Acute exposure to the respiratory system may cause coughing and irritation. Ingestion may cause gastrointestinal irritation, nausea, vomiting and diarrhoea.

What is Fuchsin reagent?

Fuchsin Acid reagent is used as a component of the Mallory Trichrome kit. The phosphotungstic acid molecule then pushes out Fuchsin Acid dye molecules from collagen and thus enables Aniline Blue to bind, resulting in collagen being stained contrast blue in relation to previously used red dye.

What is the difference between carbol Fuchsin and basic fuchsin?

Carbol fuchsin, carbol-fuchsin, or carbolfuchsin, is a mixture of phenol and basic fuchsin, used in bacterial staining procedures. Carbol fuchsin is used as the primary stain dye to detect acid-fast bacteria because it is more soluble in the cells wall lipids than in the acid alcohol.

What does Basic Fuchsin do?

The basic fuchsin is a cationic triphenylmethane dye used for the detection of acid-fast bacilli and is commonly used in the Ziehl Neelsen staining technique. It stains mucopolysaccharides and glycoproteins. It can also be used to track proteins in acidic pH systems.

Is Crystal Violet acidic or basic?

Product description: Crystal Violet is used as an acid-base indicator. When dissolved in water, the dye has a blue-violet colour with an absorbance maximum at 590nm and an extinction coefficient of 87,000 M-1 cm-1. The colour of the dye depends on the acidity of the solution.

What is Schiff’s test and its use?

The Schiff test is a chemical test used to check for the presence of aldehydes in a given analyte. This is done by reacting the analyte with a small quantity of a Schiff reagent (which is the product formed in certain dye formulation reactions such as the reaction between sodium bisulfite and fuchsin).

What stains use Carbolfuchsin?

Carbol fuchsin, carbol-fuchsin, or carbolfuchsin, is a mixture of phenol and basic fuchsin, used in bacterial staining procedures. It is commonly used in the staining of mycobacteria as it has an affinity for the mycolic acids found in their cell membranes.

Is Carbolfuchsin a Counterstain?

Our Carbol Fuchsin (Ziehl-Neelsen) Stain is used in the microscopic detection of acid-fast microorganisms such as Mycobacterium. The last step in the staining procedure is the application of the counterstain, methylene blue, which colors other cells and background material present on the slide blue.

What is hanging drop method?

Hanging drop method is the traditional method for examining the cell motility and morphology by taking the living microorganisms from the liquid media. It makes the use of glass slides with one small concave depression towards the centre, a coverslip, petroleum jelly, microbial suspension and sterile inoculating loop.