How does LysM-cre work?

LysM-Cre (MGI: 1934631) Lysozymes are antimicrobial enzymes widely produced by the innate immune cells in eukaryota. As a type of glycoside hydrolase, lysozymes function to cleave the peptidoglycan of bacterial cell wall as their antimicrobial mechanism (9).

How does CRE loxP work?

How does it work? As alluded to above, the Cre recombinase catalyzes the site specific recombination event between two loxP sites, which can be located either on the same or on separate pieces of DNA. Both 13bp repeat sequences on a single loxP site are recognized and bound by a Cre protein, forming a dimer.

What cells express LysM?

LysM activity was shown not only in myeloid cells, but also in a subset of lymphoid and erythroid cells derived from LysM-Cre positive cells [6], suggest- ing that lysozyme is also expressed in early multipotent progenitor cells at some point during development.

What does CD11b stain?

CD11b is expressed on the surface of many leukocytes including monocytes, neutrophils, natural killer cells, granulocytes and macrophages, as well as on 8% of spleen cells and 44% of bone marrow cells. Functionally, CD11b regulates leukocyte adhesion and migration to mediate the inflammatory response.

What is B220 marker?

CD19 and B220 (CD45R), an isoform of CD45, are surface markers of mature, resting B cells. CD45 is a tyrosine phosphatase that participates in the modulation of the immune response in both B and T cells. It is present on immature and mature B cells, but it is lost upon differentiation into plasma cells (1–3).

What is CD4 Cre?

CD4-Cre transgenic mice contain CD4 enhancer, promoter and silencer sequences driving the expression of a Cre recombinase gene. Cre expression commences at the very late double-negative stage and generally results in >99% deletion of loxP flanked genes by the double-positive stage of T-cell development.

What is Cre ERT2?

The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with …

What is loxP site?

LoxP sites are directional 34 bp sequences made up of two 13 bp recognition sites separated by an 8 bp spacer region. The sequences don’t occur naturally in any known genomes other than the P1 bacteriophage, and are long enough that they are unlikely to occur by chance.

Can be studied using Cre loxP system?

In particular, Cre-loxP system is widely used as an integral experimental tool for generating the conditional. This system has enabled researchers to investigate genes of interest in a tissue/cell (spatial control) and/or time (temporal control) specific manner.