How to prepare BrdU stock solution?

For a 100X stock solution, prepare BrdU at 40 mg/mL in 10 mM Tris-Cl (pH 7.6) in a 15-mL screw-cap conical tube. Place the solution in a waterbath at ~50ºC and occasionally vortex vigorously until the BrdU is completely dissolved.

Does BrdU dissolve in water?

BrdU takes up to a few hours to dissolve at 37oC depending on amount. 2. Prepare BrdU for supplementation in drinking water (if necessary). Make up solution of 0.8mg/mL BrdU in water (e.g. 0.240g in 300mL).

How do you dissolve BrdU on PBS?

For a final concentration of 100 mg BrdU / kg of animal we dissolve 33mg of BrdU in 1 ml of NaOH (Stock solution). This allow us to have more solubility.To prepare the injection solution, mix 1 part stock + 3 volumes of 0.9% NaCl in order to buffer the alkalinity (NaOH) of the stock solution.

Is BrdU toxic?

Toxicity. BrdU is a mutagen, teratogen and cytotoxin.

What is the difference between Ki67 and BrdU?

Ki67 and BrdU are two types of proliferation markers that are useful in the detection of cell proliferation. Ki67 is a specific protein, while BrdU is a synthetic nucleoside. Thus, this is the key difference between Ki67 and BrdU. Ki67 is able to label the cells in the G1, G2, S and M phases of the cell cycle.

What does BrdU stain for?

BrdU (Bromodeoxyuridine / 5-bromo-2′-deoxyuridine) is an analog of the nucleoside thymidine used in the BrdU assay to identify proliferating cells. BrdU labeling can be performed in vitro for cell lines and primary cell cultures, or in vivo for labeling cells within a living animal.

What is the difference between BrdU and EdU?

EdU assay. Unlike assays using BrdU staining, Click-iT EdU assays are not antibody-based and therefore do not require DNA denaturation for detection of the incorporated nucleoside.

Is BrdU radioactive?

Because BrdU can replace thymidine during DNA replication, it can cause mutations, and its use is therefore potentially a health hazard. However, because it is neither radioactive nor myelotoxic at labeling concentrations, it is widely preferred for in vivo studies of cancer cell proliferation.

How does BrdU labeling work?

Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. Once incorporated, these nucleoside analogs serve as cell cycle and proliferation markers that can be detected using labeled probes to identify cells that are actively proliferating.

How is BrdU used?

Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. As such, BrdU is used for birth dating and monitoring cell proliferation. As a thymidine analog, it is a marker of DNA synthesis.

Is BrdU toxic to cells?

What is the purpose of BrdU?

Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. As such, BrdU is used for birth dating and monitoring cell proliferation.

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