What are non-canonical DNA structures?
What are non-canonical DNA structures?
Non-canonical DNA and RNA segments are formed as a result of secondary structures. These include G-quadruplexes, triplex forming oligos, hairpins and cruciforms, and i-Motif structures.
What are the 4 Hydrogenous bases of DNA?
Attached to each sugar is one of four bases–adenine (A), cytosine (C), guanine (G), or thymine (T). The two strands are held together by hydrogen bonds between the bases, with adenine forming a base pair with thymine, and cytosine forming a base pair with guanine.
What is Hoogsteen base pairing and where would it be found?
This type of base pairing is found in parallel DNA discussed in Chapter 7. Hoogsteen base pairs utilize the C6–N7 face of the purine for hydrogen bonding with the Watson–Crick (N3–C4) face of the pyrimidine (Hoogsteen, 1963).
Why does DNA not form non-canonical base pairs like those found in folded RNA molecules?
Question: 17. Why does DNA not form non-canonical (i.e., non-Watson-Crick) base pairs like those found in folded RNA molecules? RNA has uracil, whereas DNA has thymine instead.
What is non-canonical?
Definition of noncanonical : not relating to, part of, or sanctioned by a canon : not canonical noncanonical literary works.
What is non Watson base pairing?
Non-canonical base pairing occurs when nucleobases hydrogen bond, or base pair, to one another in schemes other than the standard Watson-Crick base pairs (which are adenine (A) — thymine (T) in DNA, adenine (A) — uracil (U) in RNA, and guanine (G) — cytosine (C) in both DNA and RNA).
What are the 4 bases in RNA?
RNA consists of four nitrogenous bases: adenine, cytosine, uracil, and guanine. Uracil is a pyrimidine that is structurally similar to the thymine, another pyrimidine that is found in DNA. Like thymine, uracil can base-pair with adenine (Figure 2).
What is Hoogsteen edge?
Like the purine, there is the Watson-Crick edge(WC) and the Sugar edge(S) but the third edge is referred to as the “C-H” edge(H) on the pyrimidine bases. This C-H edge is sometimes also referred to as the Hoogsteen edge for simplicity. The various edges for the purine and pyrimidine bases are shown in Figure 2.
What is the difference between Watson-Crick base pairing and Hoogsteen base pairing?
In Hoogsteen base pairing, the purine base takes a different conformation with respect to pyrimidine base. So, this is the key difference between Watson and Crick and Hoogsteen base pairing. Moreover, Watson and Crick base pairs stabilize the DNA double helix while Hoogsteen base pairs make the helix unstable.
Why does non-canonical base pairing occur?