What definitive tests are used to distinguish species within the genus Staphylococcus?

What definitive tests are used to distinguish species within the genus Staphylococcus?

The major test reaction to use in Staphylococcus identification is the coagulase test reaction, which divides the genus Staphylococcus into 2 groups—coagulase negative species and coagulase positive species.

What are three specific tests have we performed in lab to identify Staphylococcus aureus?

For routine identification of Staphylococcus aureus from clinical specimens, we recommend simultaneous use of all the three tests (beginning with growth on MSA, DNase and Tube coagulase) in settings where rabbit or horse plasmas are not readily available.

What biochemical tests are used to identify Staphylococcus?

Biochemical tests used to confirm S. aureus were coagulase test, catalase test, indole production, methyl red test, Voges-proskauer reaction, urease production, citrate utilization and sugar fermentation [12].

What are the species of Staphylococcus?

The taxonomy is based on 16s rRNA sequences, and most of the staphylococcal species fall into 11 clusters:

• aureus group – S. argenteus, S.
• auricularis group – S. auricularis.
• carnosus group – S.
• epidermidis group – S.
• haemolyticus group – S.
• hyicus-intermedius group – S.
• lugdunensis group – S.
• saprophyticus group – S.

How do you identify a Staphylococcus species?

Staphylococcus aureus has traditionally been identified by tube coagulase tests that detect staphylocoagulase or “free coagulase”. However, detection of surface proteins such as clumping factor (slide coagulase test) and/or protein A (commercial latex tests) may be used for rapid identification.

Which test allows to distinguish Staphylococcus aureus from Staphylococcus saprophyticus and Staphylococcus epidermidis?

multiplex PCR
aureus and Staph. epidermidis. Accordingly, a multiplex PCR was developed and we found that a single gene encoding the adhesin fibrinogen binding protein could be used to identify and differentiate the two species.

How can you differentiate S epidermidis from S Saprophyticus?

epidermidis, another species of pathogenic CoNS, by testing for susceptibility to the antibiotic novobiocin. S. saprophyticus is novobiocin-resistant, whereas S. epidermidis is novobiocin-sensitive.

What is DNase test used for?

The test is used to determine the ability of an organism to hydrolyze DNA. DNase agar is a differential medium that tests the ability of an organism to produce an exo-enzyme, called deoxyribonuclease. DNase are extracellular endonucleases that cleave DNA and release free nucleotides and phosphate.

Who identified Staphylococcus aureus?

STAPHYLOCOCCUS IS IDENTIFIED AS A CAUSE of wound infection by the Scottish surgeon Sir Alexander Ogston, who named it for the grape-like clusters (in Greek, staphyle) he observed under the microscope. STAPHYLOCOCCUS AUREUS IS ISOLATED BY German scientist Anton Rosenbach, who grows the two strains, S.

What species does Staphylococcus aureus belong to?

Staphylococcus is a genus of Gram-positive bacteria in the family Staphylococcaceae from the order Bacillales….

What is the best test for Staphylococcus aureus infection?

Background The ideal identification of Staphylococcus aureus clinical isolates requires a battery of tests and this is costly in resource limited settings. In many developing countries, the tube coagulase test is usually confirmatory for S. aureus and is routinely done using either human or sheep plasma.

Is Staphylococcus aureus a commensal?

Background Staphylococcus aureus is a ubiquitous commensal bacterium on human skins and anterior nares, but frequently causes severe infections in humans [1].

How accurate is the coagulase test for Staphylococcus?

The sensitivity and specificity of the tube coagulase test (human plasma) was markedly improved when Mannitol salt agar and DNase were introduced as a tri-combination test for routine identification of Staphylococcus aureus (100% specificity and 75% sensitivity).