What is superficially porous particles?

Superficially porous particles (also called: shell, fused-core™, core-shell™, partially porous, pellicular) are made of a solid, non-porous core surrounded by a shell of a porous material that has properties similar to those of the fully porous materials conventionally used in HPLC.

What is LC particle?

These particles feature a solid, impermeable core enveloped by a thin, porous layer of silica that offers significantly higher efficiency and sensitivity than traditional fully porous particles. However, the best LC particle choice may not always be clear.

Why are HPLC particles porous?

Theory and experiments have clearly established that particles used for the HPLC separation of molecules should have pores that are sufficiently large to allow free movement of such molecules within the pore structure and not restrict diffusion [1, 2].

What is SPPs particle What is the advantage of using SPPs as the column packing material?

Columns packed with superficially porous particles (SPPs) have created considerable excitement over the last few years. Indeed, this column technology manifests the advantages of fully porous material (loading capacity, retention) and some beneficial properties of nonporous particles (kinetic performance).

What is column pore size?

Pore size is the average size of a pore in a porous packing. Its value is typically expressed in angstroms. The pore size determines whether a molecule can diffuse into and out of the packing.

How do I select a column in HPLC?

Picking the Perfect HPLC Column

Separation modes. Your first task in picking a column is selecting a separation chemistry.
Column length. After you’ve decided on a column type, consider its length.
Size and type of column particles.
Throughput.

What are porous particles?

Porous particles (PPs) are a novel kind of carrier with a large surface area, high porosity, stable uniform porous structure, tunable pore sizes with narrow distribution, and well defined surface properties.

What is the difference between pellicular and porous particles?

Pellicular particles are surrounded by a thin uniform layer of silica, polystyrene-divinyl-benzene synthetic resin, alumina, or other type of ion-exchange resin. The diameter of the pellicular beads is between 30 and 40 µm. Porous particles are more commonly used and have diameters between 3 to 10 µm.

What are the different types of core-shell particles?

There are a number of core–shell particles commercially available, the main ones being Poroshell (Agilent), Halo (Advanced Materials Technology), Cortecs (Waters), Kinetex (Phenomenex) and Accucore (Thermo Fisher Scientific).

How does the size of silica particles affect the column performance?

The size of silica particles and the packing quality can significantly affect the performance of the packed columns. Monodisperse silica particles with smaller diameters are employed to achieve high performance separation. However, coming with the use of smaller particles is the considerably increased back pressure [8].